Implementation of the CRISPR-Cas9 system in fission yeast.
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ABSTRACT:
SUBMITTER: Jacobs JZ
PROVIDER: S-EPMC4215166 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Implementation of the CRISPR-Cas9 system in fission yeast.
Jacobs Jake Z JZ Ciccaglione Keith M KM Tournier Vincent V Zaratiegui Mikel M
Nature communications 20141029
Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Together with constitutive expression of Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rap ...[more]
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