General approach for characterizing in vitro selected peptides with protein binding affinity.
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ABSTRACT:
SUBMITTER: Larsen AC
PROVIDER: S-EPMC4215864 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
General approach for characterizing in vitro selected peptides with protein binding affinity.
Larsen Andrew C AC Gillig Annabelle A Shah Pankti P Sau Sujay P SP Fenton Kathryn E KE Chaput John C JC
Analytical chemistry 20140714 15
In vitro selection technologies are important tools for identifying high affinity peptides to proteins of broad medical and biological interest. However, the technological advances that have made it possible to generate long lists of candidate peptides have far outpaced our ability to characterize the binding properties of individual peptides. Here, we describe a low cost strategy to rapidly synthesize, purify, screen, and characterize peptides for high binding affinity. Peptides are assayed in ...[more]
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