Construction of a Synthetically Engineered nirB Promoter for Expression of Recombinant Protein in Escherichia coli.

ABSTRACT:

Background

Anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. The nirB promoter is the promoter of the first gene of nir operon in Escherichia coli, which encodes NADH-dependent nitrite reductase. This promoter is naturally induced under anaerobic conditions and upregulated by nitrite and nitrate.

Objectives

The current study was carried out to construct a synthetic nirB promoter that does not respond to chemical inducers (nitrite or nitrate), but instead responds to anaerobic induction. For this purpose, a new plasmid was constructed (pFSnirB78-23LTB), which contains a synthetic nirB promoter. The activi

SUBMITTER: Nasr R 

PROVIDER: S-EPMC4216584 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature