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Time-resolved crystallography using the Hadamard transform.

ABSTRACT: We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.

SUBMITTER: Yorke BA

PROVIDER: S-EPMC4216935 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Time-resolved crystallography using the Hadamard transform.

Yorke Briony A BA Beddard Godfrey S GS Owen Robin L RL Pearson Arwen R AR

Nature methods 20141005 11

We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is a ...[more]

PMID: 25282611

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