A putative pH-dependent nuclear localization signal in the juxtamembrane region of c-Met.

ABSTRACT: The C-terminal fragment of the c-Met receptor tyrosine kinase is present in the nuclei of some cells irrespective of ligand stimulation, but the responsible nuclear localization signal (NLS) has not been previously reported. Here, we report that two histidine residues separated by a 10-amino-acid spacer (H1068-H1079) located in the juxtamembrane region of c-Met function as a putative novel NLS. Deletion of these sequences significantly abolished the nuclear translocation of c-Met, as did substitution of the histidines with alanines. This substitution also decreased the association of c-Met fragment with importin β. The putative NLS of c-Met is unique in that it relies on histidines, whose positive charge changes depending on pH, rather than the lysines or arginines, commonly found in class

SUBMITTER: Chaudhary SC 

PROVIDER: S-EPMC4221695 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature