Dataset Information

Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth.

ABSTRACT:

Background

There is a paucity of information regarding development of fruit tissue microstructure and changes in the cell walls during fruit growth, and how these developmental processes differ between cultivars with contrasting softening behaviour. In this study we compare two apple cultivars that show different softening rates during fruit development and ripening. We investigate whether these different softening behaviours manifest themselves late during ethylene-induced softening in the ripening phase, or early during fruit expansion and maturation.

Results

'Scifresh' (slow softening) and 'Royal Gala' (rapid softening) apples show differences in cortical microstructure and cell adhesion as early as the cell expansion phase. 'Scifresh' apples showed reduced loss of firmness and greater dry matter accumulation compared with 'Royal Gala' during early fruit development, suggesting differences in resource allocation that influence tissue structural properties. Tricellular junctions in 'Scifresh' were rich in highly-esterified pectin, contributing to stronger cell adhesion and an increased resistance to the development of large airspaces during cell expansion. Consequently, mature fruit of 'Scifresh' showed larger, more angular shaped cells than 'Royal Gala', with less airspaces and denser tissue. Stronger cell adhesion in ripe 'Scifresh' resulted in tissue fracture by cell rupture rather than by cell-to-cell-separation as seen in 'Royal Gala'. CDTA-soluble pectin differed in both cultivars during development, implicating its involvement in cell adhesion. Low pectin methylesterase activity during early stages of fruit development coupled with the lack of immuno-detectable PG was associated with increased cell adhesion in 'Scifresh'.

Conclusions

Our results indicate that cell wall structures leading to differences in softening rates of apple fruit develop early during fruit growth and well before the induction of the ripening process.

SUBMITTER: Ng JK

PROVIDER: S-EPMC4225529 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Publications

Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth.

Ng Jovyn K T JK Schröder Roswitha R Sutherland Paul W PW Hallett Ian C IC Hall Miriam I MI Prakash Roneel R Smith Bronwen G BG Melton Laurence D LD Johnston Jason W JW

BMC plant biology 20131119

<h4>Background</h4>There is a paucity of information regarding development of fruit tissue microstructure and changes in the cell walls during fruit growth, and how these developmental processes differ between cultivars with contrasting softening behaviour. In this study we compare two apple cultivars that show different softening rates during fruit development and ripening. We investigate whether these different softening behaviours manifest themselves late during ethylene-induced softening in ...[more]

PMID: 24252512

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Project description:Photooxidative stress, when combined with elevated temperatures, triggers various defense mechanisms leading to physiological, biochemical, and morphological changes in fruit tissue. Furthermore, during sun damage, apple fruit undergo textural changes characterized by high flesh firmness compared to unexposed fruit. Fuji and Royal Gala apples were suddenly exposed to sunlight on the tree and then sampled for up to 29 days. Cell wall components and lignin biosynthetic pathway analyses were carried out on the fruit tissue. At harvest, Fuji apples with different sun exposure levels, such as exposed to direct sunlight (Exp), shaded (Non-Exp), and with severe sun damage (Sev), were also characterized. In fruit suddenly exposed to sunlight, the expression levels of phenylpropanoid-related genes, phenylalanine ammonia lyase (MdPAL), chalcone synthase (MdCHS), and flavanone-3-hydroxylase (MdF3H), were upregulated in the skin and flesh of Exp and Sev. Exposure had little effect on the lignin-related genes caffeic acid O-methyltransferase 1 (MdCOMT1) and cinnamyl alcohol dehydrogenase (MdCAD) in the skin; however, the expression of these genes was highly induced in the flesh of Exp and Sev in both cultivars. Lignin deposition increased significantly in skin with sun injury (Sev); in flesh, this increase occurred late during the stress treatment. Additionally, the ethylene biosynthesis genes 1-aminocyclopropane-1-carboxylate synthase (MdACS) and 1-aminocyclopropane-1-carboxylate oxidase (MdACO) were highly expressed in the skin and flesh tissues but were more upregulated in Sev than in Exp during the time-course experiment, which paralleled the induction of the phenylpropanoid pathway and lignin accumulation. At harvest, flesh from Sev fruit exhibited higher firmness than that from Non-Exp and Exp fruit, although no differences were observed in the alcohol-insoluble residues (AIR) among groups. The fractionation of cell wall polymers revealed an increase in the uronic acid contents of the water-soluble pectin fraction (WSF) in Exp and Sev tissues compared to Non-Exp tissues, while the other pectin-rich fractions, that is, CDTA-soluble (CSF) and Na2CO3-soluble (NSF), were increased only in Sev. The amount of hemicellulose and cellulose did not differ among fruit conditions. These findings suggest that increases in the flesh firmness of apples can be promoted by photooxidative stress, which is associated with the induction of lignin accumulation in the skin and flesh of stressed fruit, with the involvement of stress phytohormones such as ethylene.

Project description:KEY MESSAGE:Comparative ultrastructural developmental time-course analysis has identified discrete stages at which the fruit plastids undergo structural and consequently functional transitions to facilitate subsequent development-guided understanding of the complex plastid biology. Plastids are the defining organelle for a plant cell and are critical for myriad metabolic functions. The role of leaf plastid, chloroplast, is extensively documented; however, fruit plastids-chromoplasts-are poorly understood, especially in the context of the diverse metabolic processes operating in these diverse plant organs. Recently, in a comparative study of the predicted plastid-targeted proteomes across seven plant species, we reported that each plant species is predicted to harbor a unique set of plastid-targeted proteins. However, the temporal and developmental context of these processes remains unknown. In this study, an ultrastructural analysis approach was used to characterize fruit plastids in the epidermal and collenchymal cell layers at 11 developmental timepoints in three genotypes of apple (Malus × domestica Borkh.): chlorophyll-predominant 'Granny Smith', carotenoid-predominant 'Golden Delicious', and anthocyanin-predominant 'Top Red Delicious'. Plastids transitioned from a proplastid-like plastid to a chromoplast-like plastid in epidermis cells, while in the collenchyma cells, they transitioned from a chloroplast-like plastid to a chloro-chromo-amyloplast plastid. Plastids in the collenchyma cells of the three genotypes demonstrated a diverse array of structures and features. This study enabled the identification of discrete developmental stages during which specific functions are most likely being performed by the plastids as indicated by accumulation of plastoglobuli, starch granules, and other sub-organeller structures. Information regarding the metabolically active developmental stages is expected to facilitate biologically relevant omics studies to unravel the complex biochemistry of plastids in perennial non-model systems.

Dataset Information

Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth.

Background

Results

Conclusions

Publications

Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth.

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