Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
Ontology highlight
ABSTRACT: Background
Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect.Results
We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo.Conclusions
This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
SUBMITTER: Cheng H
PROVIDER: S-EPMC4226911 | biostudies-literature |
REPOSITORIES: biostudies-literature
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