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PaperClip: rapid multi-part DNA assembly from existing libraries.

ABSTRACT: Assembly of DNA 'parts' to create larger constructs is an essential enabling technique for bioengineering and synthetic biology. Here we describe a simple method, PaperClip, which allows flexible assembly of multiple DNA parts from currently existing libraries cloned in any vector. No restriction enzymes, mutagenesis of internal restriction sites, or reamplification to add end homology are required. Order of assembly is directed by double stranded oligonucleotides-'Clips'. Clips are formed by ligation of pairs of oligonucleotides corresponding to the ends of each part. PaperClip assembly can be performed by polymerase chain reaction or by cell extract-mediated recombination. Once multi-use Clips have been prepared, assembly of at least six DNA parts in any order can be accomplished with high efficiency within several hours.

SUBMITTER: Trubitsyna M

PROVIDER: S-EPMC4227759 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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PaperClip: rapid multi-part DNA assembly from existing libraries.

Trubitsyna Maryia M Michlewski Gracjan G Cai Yizhi Y Elfick Alistair A French Christopher E CE

Nucleic acids research 20140908 20

Assembly of DNA 'parts' to create larger constructs is an essential enabling technique for bioengineering and synthetic biology. Here we describe a simple method, PaperClip, which allows flexible assembly of multiple DNA parts from currently existing libraries cloned in any vector. No restriction enzymes, mutagenesis of internal restriction sites, or reamplification to add end homology are required. Order of assembly is directed by double stranded oligonucleotides-'Clips'. Clips are formed by li ...[more]

PMID: 25200084

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