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ABSTRACT:
SUBMITTER: Zhang G
PROVIDER: S-EPMC4231866 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Zhang Guofang G Yu Dan D Yang Guodong G Dong Hui H Zhang Tongcun T Liu Xiang X
Acta crystallographica. Section F, Structural biology communications 20141031 Pt 11
RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His6 tag by Ni2+-chelating affinity chromatography for X-ray crystallographic analysis. Diffraction-quality crystals were grown by the hanging-drop vapour-diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with uni ...[more]