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Quantifying local density of optical states of nanorods by fluorescence lifetime imaging.

ABSTRACT: In this letter, we demonstrate a facile far-field approach to quantify the near-field local density of optical states (LDOS) of a nanorod using CdTe quantum dots (QDs) emitters tethered to the surface of nanorods as beacons for optical read-outs. Radiative decay rate was extracted to quantify the LDOS; our analysis indicates that the LDOS of the nanorod enhance both the radiative and nonradiative decay of QD, particularly radiative decay of QDs at the end of nanorod is enhanced by 1.17 times greater than that at the waist, while the nonradiative decay was uniformly enhanced over the nanorod. To the best of our knowledge, our effort constitutes the first to map the LDOS of a nanostructure via far-field method, to provide clarity on the interaction mechanism between emitters and the nanostructure, and to be potentially employed in the LDOS mapping of high-throughput nanostructures.

SUBMITTER: Liu J

PROVIDER: S-EPMC4232967 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Quantifying local density of optical states of nanorods by fluorescence lifetime imaging.

Liu Jing J Jiang Xunpeng X Ishii Satoshi S Shalaev Vladimir V Irudayaraj Joseph J

New journal of physics 20140601

In this letter, we demonstrate a facile far-field approach to quantify the near-field local density of optical states (LDOS) of a nanorod using CdTe quantum dots (QDs) emitters tethered to the surface of nanorods as beacons for optical read-outs. Radiative decay rate was extracted to quantify the LDOS; our analysis indicates that the LDOS of the nanorod enhance both the radiative and nonradiative decay of QD, particularly radiative decay of QDs at the end of nanorod is enhanced by 1.17 times gre ...[more]

PMID: 25408619

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Project description:Two-photon laser-scanning microscopy has revolutionized our view on vital processes by revealing motility and interaction patterns of various cell subsets in hardly accessible organs (e.g. brain) in living animals. However, current technology is still insufficient to elucidate the mechanisms of organ dysfunction as a prerequisite for developing new therapeutic strategies, since it renders only sparse information about the molecular basis of cellular response within tissues in health and disease. In the context of imaging, Förster resonant energy transfer (FRET) is one of the most adequate tools to probe molecular mechanisms of cell function. As a calibration-free technique, fluorescence lifetime imaging (FLIM) is superior for quantifying FRET in vivo. Currently, its main limitation is the acquisition speed in the context of deep-tissue 3D and 4D imaging. Here we present a parallelized time-correlated single-photon counting point detector (p-TCSPC) (i) for dynamic single-beam scanning FLIM of large 3D areas on the range of hundreds of milliseconds relevant in the context of immune-induced pathologies as well as (ii) for ultrafast 2D FLIM in the range of tens of milliseconds, a scale relevant for cell physiology. We demonstrate its power in dynamic deep-tissue intravital imaging, as compared to multi-beam scanning time-gated FLIM suitable for fast data acquisition and compared to highly sensitive single-channel TCSPC adequate to detect low fluorescence signals. Using p-TCSPC, 256×256 pixel FLIM maps (300×300 µm(2)) are acquired within 468 ms while 131×131 pixel FLIM maps (75×75 µm(2)) can be acquired every 82 ms in 115 µm depth in the spinal cord of CerTN L15 mice. The CerTN L15 mice express a FRET-based Ca-biosensor in certain neuronal subsets. Our new technology allows us to perform time-lapse 3D intravital FLIM (4D FLIM) in the brain stem of CerTN L15 mice affected by experimental autoimmune encephalomyelitis and, thereby, to truly quantify neuronal dysfunction in neuroinflammation.

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Quantifying local density of optical states of nanorods by fluorescence lifetime imaging.

Publications

Quantifying local density of optical states of nanorods by fluorescence lifetime imaging.

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