Unknown

Dataset Information

0

Targeting GPR30 with G-1: a new therapeutic target for castration-resistant prostate cancer.


ABSTRACT: Castration-resistant prostate cancer (CRPC) is an advanced-stage prostate cancer (PC) associated with high mortality. We reported that G-1, a selective agonist of G protein-coupled receptor 30 (GPR30), inhibited PC cell growth by inducing G2 cell cycle arrest and arrested PC-3 xenograft growth. However, the therapeutic actions of G-1 and their relationships with androgen in vivo are unclear. Using the LNCaP xenograft to model PC growth during the androgen-sensitive (AS) versus the castration-resistant (CR) phase, we found that G-1 inhibited growth of CR but not AS tumors with no observable toxicity to the host. Substantial necrosis (approximately 65%) accompanied by marked intratumoral infiltration of neutrophils was observed only in CR tumors. Global transcriptome profiling of human genes identified 99 differentially expressed genes with 'interplay between innate and adaptive immune responses' as the top pathway. Quantitative PCR confirmed upregulation of neutrophil-related chemokines and inflammation-mediated cytokines only in the G-1-treated CR tumors. Expression of murine neutrophil-related cytokines also was elevated in these tumors. GPR30 (GPER1) expression was significantly higher in CR tumors than in AS tumors. In cell-based experiments, androgen repressed GPR30 expression, a response reversible by anti-androgen or siRNA-induced androgen receptor silencing. Finally, in clinical specimens, 80% of CRPC metastases (n=123) expressed a high level of GPR30, whereas only 54% of the primary PCs (n=232) showed high GPR30 expression. Together, these results provide the first evidence, to our knowledge, that GPR30 is an androgen-repressed target and G-1 mediates the anti-tumor effect via neutrophil-infiltration-associated necrosis in CRPC. Additional studies are warranted to firmly establish GPR30 as a therapeutic target in CRPC.

SUBMITTER: Lam HM 

PROVIDER: S-EPMC4233119 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Targeting GPR30 with G-1: a new therapeutic target for castration-resistant prostate cancer.

Lam Hung-Ming HM   Ouyang Bin B   Chen Jing J   Ying Jun J   Wang Jiang J   Wu Chin-Lee CL   Jia Li L   Medvedovic Mario M   Vessella Robert L RL   Ho Shuk-Mei SM  

Endocrine-related cancer 20141006 6


Castration-resistant prostate cancer (CRPC) is an advanced-stage prostate cancer (PC) associated with high mortality. We reported that G-1, a selective agonist of G protein-coupled receptor 30 (GPR30), inhibited PC cell growth by inducing G2 cell cycle arrest and arrested PC-3 xenograft growth. However, the therapeutic actions of G-1 and their relationships with androgen in vivo are unclear. Using the LNCaP xenograft to model PC growth during the androgen-sensitive (AS) versus the castration-res  ...[more]

Similar Datasets

| S-EPMC3519986 | biostudies-literature
| S-EPMC3228074 | biostudies-literature
| S-EPMC4075966 | biostudies-literature
| S-EPMC7079209 | biostudies-literature
2014-04-25 | E-GEOD-55064 | biostudies-arrayexpress
| S-EPMC6853839 | biostudies-literature
| S-EPMC7864378 | biostudies-literature
| S-EPMC3562731 | biostudies-literature
2014-04-25 | E-GEOD-55063 | biostudies-arrayexpress
2014-04-25 | GSE55064 | GEO