Dataset Information

A cytosolic glucose-6-phosphate dehydrogenase gene, ScG6PDH, plays a positive role in response to various abiotic stresses in sugarcane.

ABSTRACT: As one of the key enzymes in the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) plays a role in response to abiotic stresses and pathogenesis. Here, a full-length cDNA was obtained, designed as ScG6PDH from sugarcane. The ScG6PDH gene is 1,646 bp long with a 1,524-bp long ORF encoding 507 amino acid residues. Analysis of a phylogenetic tree indicated that this gene is a member of the cytosolic G6PDH gene family, which is consistent with results from a subcellular localization experiment. Based on a real-time quantitative RT-PCR performed under salt, drought, heavy metal (CdCl2) and low temperature (4 °C) treatments, the transcription levels of the ScG6PDH gene were higher compared with transcription levels where these treatments were not imposed, suggesting a positive response of this gene to these environmental stresses. Furthermore, G6PDH activity was stimulated under 4 °C, CdCl2, NaCl and PEG treatments, but the increments varied with treatment and sampling time, implying positive response to abiotic stresses, similar to the transcript of the G6PDH gene. Ion conductivity measurements and a histochemical assay provided indirect evidence of the involvement of the ScG6PDH gene in defense reactions to the above-mentioned abiotic stresses.

SUBMITTER: Yang Y

PROVIDER: S-EPMC4235309 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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A cytosolic glucose-6-phosphate dehydrogenase gene, ScG6PDH, plays a positive role in response to various abiotic stresses in sugarcane.

Yang Yuting Y Fu Zhiwei Z Su Yachun Y Zhang Xu X Li Guoyin G Guo Jinlong J Que Youxiong Y Xu Liping L

Scientific reports 20141118

As one of the key enzymes in the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH) plays a role in response to abiotic stresses and pathogenesis. Here, a full-length cDNA was obtained, designed as ScG6PDH from sugarcane. The ScG6PDH gene is 1,646 bp long with a 1,524-bp long ORF encoding 507 amino acid residues. Analysis of a phylogenetic tree indicated that this gene is a member of the cytosolic G6PDH gene family, which is consistent with results from a subcellular ...[more]

PMID: 25404472

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Project description:Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas), a root crop with worldwide importance. The increased production of glycine betaine (GB) improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH) is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH) was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS) production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait improvement in sweet potato not only stabilizes yield production in normal soils in unpredictable climates but also provides a novel germplasm for sweet potato production on marginal lands.

Project description:Background and Aims:Root knot nematodes (RKNs, Meloidogyne spp.) are microscopic roundworms with a wide host range causing great economic losses worldwide. Understanding how metabolic pathways function within the plant upon RKN infection will provide insight into the molecular aspects of plant-RKN interactions. Glucose-6-phosphate dehydrogenase (G6PDH), the key regulatory enzyme of the oxidative pentose phosphate pathway (OPPP), is involved in plant responses to abiotic stresses and pathogenesis. In this study, the roles of Arabidopsis cytosolic G6PDH in plant-RKN interactions were investigated. Methods:Enzyme assays and western blotting were used to characterize changes in total G6PDH activity and protein abundance in wild-type Arabidopsis in response to RKN infection. The susceptibility of wild-type plants and the double mutant g6pd5/6 to RKNs was analysed and the expression of genes associated with the basal defence response was tested after RKN infection using quantitative reverse transcription PCR. Key Results:RKN infection caused a marked increase in total G6PDH activity and protein abundance in wild-type Arabidopsis roots. However, the transcript levels of G6PDH genes except G6PD6 were not significantly induced following RKN infection, suggesting that the increase in G6PDH activity may occur at the post-transcriptional level. The double mutant g6pd5/6 with loss-of-function of the two cytosolic isoforms G6PD5 and G6PD6 displayed enhanced susceptibility to RKNs. Moreover, reactive oxygen species (ROS) production and gene expression involved in the defence response including jasmonic acid and salicylic acid pathways were suppressed in the g6pd5/6 mutant at the early stage of RKN infection when compared to the wild-type plants. Conclusions:The results demonstrated that the G6PDH-mediated OPPP plays an important role in the plant-RKN interaction. In addition, a new aspect of G6PDH activity involving NADPH production by the OPPP in plant basal defence against RKNs is defined, which may be involved in ROS signalling.

Dataset Information

A cytosolic glucose-6-phosphate dehydrogenase gene, ScG6PDH, plays a positive role in response to various abiotic stresses in sugarcane.

Publications

A cytosolic glucose-6-phosphate dehydrogenase gene, ScG6PDH, plays a positive role in response to various abiotic stresses in sugarcane.

Similar Datasets

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