Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro.

ABSTRACT: The effect of biomaterial topography on healing in vivo and monocyte/macrophage stimulation in vitro was assessed. A series of expanded polytetrafluoroethylene (ePTFE) materials were characterized by increasing average intranodal distance of 1.2 μm (1.2-ePTFE), 3.0 μm (3.0-ePTFE), and 4.4 μm (4.4-ePTFE), but presented consistent surface chemistry with nonporous PTFE (np-PTFE). Subcutaneous implantation of 4.4-ePTFE into mice resulted in a statistically thinner capsule that appeared less organized and less dense than the np-PTFE response. In vitro, isolated monocytes/macrophages cultured on np-PTFE produced low levels of interleukin 1-beta (IL-1β), 1.2-ePTFE and 3.0-ePTFE stimulated intermediate levels, and 4.4-ePTFE stimulated a 15-fold increase over np-PTFE. Analysis of cDNA microarrays d

SUBMITTER: Bota PC 

PROVIDER: S-EPMC4235956 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature