Dataset Information

Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia.

ABSTRACT: Our aim is to detect the infection by Toscana virus (TOSV) and other Phleboviruses in the sera and cerebro-spinal fluid (CSF) of patients with meningitis in Tunisia. We examined various species of phlebotomus present in Tunisia to determine whether or not a direct relationship exists between cases of meningitis and the viruses circulating in the insect vectors.Patients with the meningeal syndrome were tested for anti-TOSV IgM and IgG using an indirect Enzyme-Linked Immunosorbent Assay (ELISA) and for the presence of TOSV and other Phleboviruses using a RT-PCR test.Of 263 patients were tested using ELISA of which 12.16% (n?=?32/263) were IgM positive for anti TOSV. Of these 32 patients, 78% (n?=?25/32) were IgG positive. 12.86% (n?=?18/140) of the CSF samples tested by RT-PCR were positive for the Toscana virus.This study confirms, for the first time, that TOSV is involved in a neurological disorder in North Africa. The incidence of this involvement in Tunisia conforms with observations made in other Mediterranean countries. Moreover, for the first time, a molecular approach was used to detect SFSV in a Tunisian patient displaying neurological symptoms.

SUBMITTER: Fezaa O

PROVIDER: S-EPMC4236749 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia.

Fezaa Ons O M'ghirbi Youmna Y Savellini Gianni Gori GG Ammari Lamia L Hogga Nahed N Triki Henda H Cusi Maria Grazia MG Bouattour Ali A

BMC infectious diseases 20141115

<h4>Background</h4>Our aim is to detect the infection by Toscana virus (TOSV) and other Phleboviruses in the sera and cerebro-spinal fluid (CSF) of patients with meningitis in Tunisia. We examined various species of phlebotomus present in Tunisia to determine whether or not a direct relationship exists between cases of meningitis and the viruses circulating in the insect vectors.<h4>Methods</h4>Patients with the meningeal syndrome were tested for anti-TOSV IgM and IgG using an indirect Enzyme-Li ...[more]

PMID: 25398483

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Project description:BackgroundBovine babesiosis, mainly caused by Babesia bovis and B. bigemina, is a huge threat to the livestock industry. In Indonesia, the current distribution of the disease is unknown due to a lack of scientific study.MethodsIn the present study, 487 blood samples were collected from cattle with different breeding and age groups in a broad geographical area across the archipelago. The presence of antibodies and current infections of B. bovis and B. bigemina were determined using enzyme-linked immunosorbent assay (ELISA), immunochromatographic test (ICT), and nested PCR (nPCR) targeting B. bovis SBP-4 and B. bigemina RAP-1a genes. Sequence analysis was performed to the amplicon of B. bovis SBP-4, B. bigemina RAP-1a, and internal transcribed spacer (ITS) region of ribosomal RNA of both Babesia species.ResultsIn total, B. bovis positives were detected by ELISA, single-ICT, dual-ICT and nPCR in 340 (69.8%), 317 (65.1%), 307 (63.0%) and 247 (50.7%) samples, respectively. For B. bigemina, the positive samples were detected in 134 (27.5%), 130 (26.7%), 127 (26.1%) and 93 (19.1%), respectively. Furthermore, mixed infections were found in 125 (25.7%), 113 (23.2%), 109 (22.4%) and 52 (10.7%) samples, respectively, which occurred only by chance and were not influenced by additional factors. The obtained nucleotide sequences of B. bovis SBP-4 and B. bigemina RAP-1a genes showed a high homology with other isolates from different countries. Further nucleotide sequence analysis using ITS region showed a great genetic diversity of B. bovis isolates among sampling locations; a lower diversity was found in B. bigemina ITS isolates.ConclusionsThese data revealed the current distribution of B. bovis and B. bigemina infection in cattle in Indonesia. The rate of infection varied among sampling locations, cattle breeds and age groups. Furthermore, B. bovis ITS isolates from Indonesia were found to be more genetically diverse than B. bigemina ITS isolates. The data presented in this study are necessary to develop an effective strategy for controlling the disease in the country.

Dataset Information

Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia.

Publications

Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia.

Similar Datasets

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