Dataset Information

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

ABSTRACT:

Background

Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs. Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.

Methods

In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.

Results

Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of 6 to 7 kDa peptides and proved to be a highly practical peptidomics tool with better resolution when compared to conventional polyacrylamide gel electrophoresis. Mass spectroscopy analysis helped us to identify 45 unique peptide masses between 1 to 7 kDa with a bimodal mass distribution peaking between molecular weights of 1 to 2 kDa (29%) and 3 to 4 kDa (31%). L. abdullahbayrami crude venom had a proliferative effect on MCF-7 cells, which may be explained by the high concentration of polyamines as well as potassium and calcium ions in the arachnid venoms. Antimicrobial effect was stronger on gram-negative bacteria.

Conclusions

This work represents the first peptidomic characterization of L. abdullahbayrami venom. Considering the molecular weight-function relationship of previously identified venom peptides, future bioactivity studies may lead to the discovery of novel potassium and chloride ion channel inhibitors as well as new antimicrobial peptides from L. abdullahbayrami venom.

SUBMITTER: Erdes E

PROVIDER: S-EPMC4237746 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

Erdeş Efe E Doğan Tuğba Somay TS Coşar Ilhan I Danışman Tarık T Kunt Kadir Boğaç KB Seker Tamay T Yücel Meral M Ozen Can C

The journal of venomous animals and toxins including tropical diseases 20141103 1

<h4>Background</h4>Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs. Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.<h4>Methods</h4>In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxi ...[more]

PMID: 25414725

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Project description:Since the publication of the ground-breaking "Catalogue of the scorpions of the world (1758-1998)" (Fet et al. 2000) the number of species in the scorpion genus Buthus Leach, 1815 has increased 10-fold, and this genus is now the fourth largest within the Buthidae, with 52 valid named species. Here we revise and update the available information regarding Buthus. A new combination is proposed: Buthus halius (C. L. Koch, 1839), comb. n. from Portugal and Spain. B. halius is removed from junior synonymy with Buthus occitanus (Amoreux, 1789), and proposed as a senior synonym of B. ibericus Lourenço & Vachon, 2004, syn. n. Moreover, following I.C.Z.N. article 23.9.2 we propose to maintain as valid B. ibericus(nomen protectum) and to consider the disued B. halius as a nomen oblitum . Buthus europaeus tridentatus Franganillo, 1918 is proposed as a junior synonym of B. occitanus (Amoreux, 1789), syn. n.Buthus sabulicola Touloun, 2012 is proposed as a junior synonym of Buthus bonito Lourenço & Geniez, 2005, syn. n.Buthus occitanus tunetanus neeli Gysin, 1969 is proposed as an informal senior synonym of Buthus tassili Lourenço, 2002, informal syn. n. Two taxa are rised to species rank, Buthus nigrovesiculosus Hirst, 1925, stat. n. and Buthus parroti Vachon, 1949, stat. n.. We further confirm the restricted distribution of B. occitanus that is confined to southeastern France and northwestern Iberian Peninsula and does not occur in North Africa. Additionally, Androctonus barbouri (Werner, 1932), comb. n. from the Agadir region of Morocco, is hereby transferred to the genus Androctonus. We summarize and provide a critical appraisal of the diagnostic characters currently in use for the genus. The catalogue section considers the names for species, subspecies and varieties that have been used for Buthus scorpions. Information about types, including collection numbers and localities are included when available. Finally, an annotated listing of synonymies and an updated bibliography are given.

Dataset Information

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

Background

Methods

Results

Conclusions

Publications

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

Similar Datasets

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