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Stable small quantum dots for synaptic receptor tracking on live neurons.

ABSTRACT: We developed a coating method to produce functionalized small quantum dots (sQDs), about 9?nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655?nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

SUBMITTER: Cai E

PROVIDER: S-EPMC4240739 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Stable small quantum dots for synaptic receptor tracking on live neurons.

Cai En E Ge Pinghua P Lee Sang Hak SH Jeyifous Okunola O Wang Yong Y Liu Yanxin Y Wilson Katie M KM Lim Sung Jun SJ Baird Michelle A MA Stone John E JE Lee Kwan Young KY Davidson Michael W MW Chung Hee Jung HJ Schulten Klaus K Smith Andrew M AM Green William N WN Selvin Paul R PR

Angewandte Chemie (International ed. in English) 20140926 46

We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs. ...[more]

PMID: 25255882

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