Ontology highlight
ABSTRACT:
SUBMITTER: Guerrero-Ferreira RC
PROVIDER: S-EPMC4240974 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Guerrero-Ferreira Ricardo C RC Wright Elizabeth R ER
Journal of structural biology 20130925 1
Cryo-electron tomography (cryo-ET) provides three-dimensional (3D) structural information of bacteria preserved in a native, frozen-hydrated state. The typical low contrast of tilt-series images, a result of both the need for a low electron dose and the use of conventional defocus phase-contrast imaging, is a challenge for high-quality tomograms. We show that Zernike phase-contrast imaging allows the electron dose to be reduced. This limits movement of gold fiducials during the tilt series, whic ...[more]