NMR characterization of the conformational fluctuations of the human lymphocyte function-associated antigen-1 I-domain.

ABSTRACT: Lymphocyte function-associated antigen-1 (LFA-1) is an integrin protein that transmits information across the plasma membrane through the so-called inside-out and outside-in signaling mechanisms. To investigate these mechanisms, we carried out an NMR analysis of the dynamics of the LFA-1 I-domain, which has enabled us to characterize the motions of this domain on a broad range of timescales. We studied first the internal motions on the nanosecond timescale by spin relaxation measurements and model-free analysis. We then extended this analysis to the millisecond timescale motions by measuring (15) N-(1) H residual dipolar couplings of the backbone amide groups. We analyzed these results in the context of the three major conformational states of the I-domain using their corresponding X-ray c

SUBMITTER: Leung HT 

PROVIDER: S-EPMC4241110 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature