Dataset Information

Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at cahuil lagoon, chile.

ABSTRACT: Cáhuil Lagoon in central Chile harbors distinct microbial communities in various solar salterns that are arranged as interconnected ponds with increasing salt concentrations. Here, we report the metagenome of the 3.0- to 0.2-µm fraction of the microbial community present in a crystallizer pond with 34% salinity.

SUBMITTER: Plominsky AM

PROVIDER: S-EPMC4241667 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at cáhuil lagoon, chile.

Plominsky Alvaro M AM Delherbe Nathalie N Ugalde Juan A JA Allen Eric E EE Blanchet Marine M Ikeda Priscila P Santibañez Francisco F Hanselmann Kurt K Ulloa Osvaldo O De la Iglesia Rodrigo R von Dassow Peter P Astorga Marcia M Gálvez María Jesús MJ González María Lorena ML Henríquez-Castillo Carlos C Vaulot Daniel D Lopes do Santos Adriana A van den Engh Gerrit G Gimpel Carla C Bertoglio Florencia F Delgado Yolaine Y Docmac Felipe F Elizondo-Patrone Claudia C Narváez Silvia S Sorroche Fernando F Rojas-Herrera Marcelo M Trefault Nicole N

Genome announcements 20141113 6

Cáhuil Lagoon in central Chile harbors distinct microbial communities in various solar salterns that are arranged as interconnected ponds with increasing salt concentrations. Here, we report the metagenome of the 3.0- to 0.2-µm fraction of the microbial community present in a crystallizer pond with 34% salinity. ...[more]

PMID: 25395641

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Project description:The paucity of sequence data from pelagic deep-ocean microbial assemblages has severely restricted molecular exploration of the largest biome on Earth. In this study, an analysis is presented of a large-scale 454-pyrosequencing metagenomic dataset from a hadopelagic environment from 6,000 m depth within the Puerto Rico Trench (PRT). A total of 145 Mbp of assembled sequence data was generated and compared to two pelagic deep ocean metagenomes and two representative surface seawater datasets from the Sargasso Sea. In a number of instances, all three deep metagenomes displayed similar trends, but were most magnified in the PRT, including enrichment in functions for two-component signal transduction mechanisms and transcriptional regulation. Overrepresented transporters in the PRT metagenome included outer membrane porins, diverse cation transporters, and di- and tri-carboxylate transporters that matched well with the prevailing catabolic processes such as butanoate, glyoxylate and dicarboxylate metabolism. A surprisingly high abundance of sulfatases for the degradation of sulfated polysaccharides were also present in the PRT. The most dramatic adaptational feature of the PRT microbes appears to be heavy metal resistance, as reflected in the large numbers of transporters present for their removal. As a complement to the metagenome approach, single-cell genomic techniques were utilized to generate partial whole-genome sequence data from four uncultivated cells from members of the dominant phyla within the PRT, Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Planctomycetes. The single-cell sequence data provided genomic context for many of the highly abundant functional attributes identified from the PRT metagenome, as well as recruiting heavily the PRT metagenomic sequence data compared to 172 available reference marine genomes. Through these multifaceted sequence approaches, new insights have been provided into the unique functional attributes present in microbes residing in a deeper layer of the ocean far removed from the more productive sun-drenched zones above.

Dataset Information

Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at cahuil lagoon, chile.

Publications

Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at cáhuil lagoon, chile.

Similar Datasets

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