Ontology highlight
ABSTRACT:
SUBMITTER: Gopalasubramaniam SK
PROVIDER: S-EPMC4243682 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Gopalasubramaniam Sabarinathan K SK Kondapalli Kalyan C KC Millán-Pacheco César C Pastor Nina N Stemmler Timothy L TL Moran Jose F JF Arredondo-Peter Raúl R
Sciencejet 20130101
Ferrous oxygenated hemoglobins (Hb<sup>2+</sup>O<sub>2</sub>) autoxidize to ferric Hb<sup>3+</sup>, but Hb<sup>3+</sup> is reduced to Hb<sup>2+</sup> by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb1<sup>3+</sup>). Spectroscopic analysis showed that FLbR2 reduces Hb1<sup>3+</sup>. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb1<sup>3+</s ...[more]