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In-house development of an optimized synthetic module for routine [11C]acetate production.

ABSTRACT: [11C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[18F]fluoro-D-glucose in a number of cancers. Here, the optimized automatic synthesis of [11C]acetate using an in-house-developed module under different conditions has been reported for routine production. [11C]CO2 was produced in a 16.4?MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [11C]acetate. The total reaction time of [11C]acetate was within 15?min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [11C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [11C]acetate produced were appropriate for clinical PET study.

SUBMITTER: Jang HY

PROVIDER: S-EPMC4243701 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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In-house development of an optimized synthetic module for routine [11C]acetate production.

Jang Hwa Youn HY Kwon Seong Young SY Pyo Ayoung A Hur Min Goo MG Kim Sang Wook SW Park Jeong-Hoon JH Kim Hee-Jung HJ Yang Seung Dae SD Lee Sunwoo S Kim Dong-Yeon DY Min Jung-Joon JJ

Nuclear medicine communications 20150101 1

[11C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[18F]fluoro-D-glucose in a number of cancers. Here, the optimized automatic synthesis of [11C]acetate using an in-house-developed module under different conditions has been reported for routine production. [11C]CO2 was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction ...[more]

PMID: 25244351

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In-house development of an optimized synthetic module for routine [11C]acetate production.

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In-house development of an optimized synthetic module for routine [11C]acetate production.

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