Epigenetic changes of mesenchymal stem cells in three-dimensional (3D) spheroids.
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ABSTRACT:
SUBMITTER: Guo L
PROVIDER: S-EPMC4244016 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Epigenetic changes of mesenchymal stem cells in three-dimensional (3D) spheroids.
Guo Ling L Zhou Ying Y Wang Shan S Wu Yaojiong Y
Journal of cellular and molecular medicine 20140805 10
Mesenchymal stem cells (MSCs) hold profound promise in tissue repair/regeneration. However, MSCs undergo remarkable spontaneous differentiation and aging during monolayer culture expansion. In this study, we found that 2-3 days of three-dimensional (3D) spheroid culture of human MSCs (hMSCs) that had been expanded in monolayer for six passages increased their clonogenicity and differentiation potency to neuronal cells. Moreover, in accordance with these changes, the expression levels of miRNA wh ...[more]
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