Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro.

ABSTRACT: The ultrasound-assisted extraction of phenolic compounds from Epimedium brevicornu Maxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X 1), extraction time (X 2), and ratio of aqueous ethanol to raw material (X 3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions are X 1 of 50% (v/v), X 2 of 27.5 min, and X 3 of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033% (n = 3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds from Epimedium brevicornu Maxim possess significant antioxidant activity. HPLC ana

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC4244914 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature