An improved surface passivation method for single-molecule studies.
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ABSTRACT:
SUBMITTER: Hua B
PROVIDER: S-EPMC4245390 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
An improved surface passivation method for single-molecule studies.
Hua Boyang B Han Kyu Young KY Zhou Ruobo R Kim Hajin H Shi Xinghua X Abeysirigunawardena Sanjaya C SC Jain Ankur A Singh Digvijay D Aggarwal Vasudha V Woodson Sarah A SA Ha Taekjip T
Nature methods 20141012 12
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations ...[more]
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