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An improved surface passivation method for single-molecule studies.

ABSTRACT: We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.

SUBMITTER: Hua B

PROVIDER: S-EPMC4245390 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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An improved surface passivation method for single-molecule studies.

Hua Boyang B Han Kyu Young KY Zhou Ruobo R Kim Hajin H Shi Xinghua X Abeysirigunawardena Sanjaya C SC Jain Ankur A Singh Digvijay D Aggarwal Vasudha V Woodson Sarah A SA Ha Taekjip T

Nature methods 20141012 12

We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations ...[more]

PMID: 25306544

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