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Energetically biased DNA motor containing a thermodynamically stable partial strand displacement state.

ABSTRACT: Current work in tuning DNA kinetics has focused on changing toehold lengths and DNA concentrations. However, kinetics can also be improved by enhancing the completion probability of the strand displacement process. Here, we execute this strategy by creating a toehold DNA motor device with the inclusion of a synthetic nucleotide, inosine, at selected sites. Furthermore, we found that the energetic bias can be tuned such that the device can stay in a stable partially displaced state. This work demonstrates the utility of energetic biases to change DNA strand displacement kinetics and introduces a complementary strategy to the existing designs.

SUBMITTER: Landon PB

PROVIDER: S-EPMC4245991 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Energetically biased DNA motor containing a thermodynamically stable partial strand displacement state.

Landon Preston B PB Lee Joon J Hwang Michael Taeyoung MT Mo Alexander H AH Zhang Chen C Neuberger Anthony A Meckes Brian B Gutierrez Jose J JJ Glinsky Gennadi G Lal Ratnesh R

Langmuir : the ACS journal of surfaces and colloids 20141111 46

Current work in tuning DNA kinetics has focused on changing toehold lengths and DNA concentrations. However, kinetics can also be improved by enhancing the completion probability of the strand displacement process. Here, we execute this strategy by creating a toehold DNA motor device with the inclusion of a synthetic nucleotide, inosine, at selected sites. Furthermore, we found that the energetic bias can be tuned such that the device can stay in a stable partially displaced state. This work dem ...[more]

PMID: 25347360

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