Ontology highlight
ABSTRACT:
SUBMITTER: Wei Y
PROVIDER: S-EPMC4246965 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Wei Yifeng Y Funk Michael A MA Rosado Leonardo A LA Baek Jiyeon J Drennan Catherine L CL Stubbe JoAnne J
Proceedings of the National Academy of Sciences of the United States of America 20140825 36
The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO2. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RN ...[more]