Unknown

Dataset Information

0

PHA-4/FOXA-regulated microRNA feed forward loops during Caenorhabditis elegans dietary restriction.


ABSTRACT: Dietary restriction (DR) increases life span and delays the onset of age-related diseases across species. However, the molecular mechanisms have remained relatively unexplored in terms of gene regulation. InC. elegans, a popular model for aging studies, the FOXA transcription factor PHA-4 is a robust genetic regulator of DR, although little is known about how it regulates gene expression. We profiled the transcriptome and miRNAome of an eat-2 mutant, a genetic surrogate of DR, by Next Generation sequencing and find that most of the miRNAs are upregulated in the young-adult worms, none significantly downregulated. Interestingly, PHA-4 can potentially regulate the expression of most of these miRNA genes. Remarkably, many of the PHA-4-regulated genes that are induced during DR are also targets of the PHA-4-upregulated miRNAs, forming a large feed-forward gene regulatory network. The genes targeted by the feed-forward loops (FFLs) are enriched for functions related to ubiquitin-mediated decay, lysosomal autophagy, cellular signalling, protein folding etc., processes that play critical roles in DR and longevity. Together our data provides a framework for understanding the complex and unique regulatory network employed during DR, suggesting that PHA-4 employs such FFLs to fine-tune gene expression and instil robustness in the system during energy crisis.

SUBMITTER: Pandit A 

PROVIDER: S-EPMC4247386 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

PHA-4/FOXA-regulated microRNA feed forward loops during Caenorhabditis elegans dietary restriction.

Pandit Awadhesh A   Jain Vaibhav V   Kumar Neeraj N   Mukhopadhyay Arnab A  

Aging 20141001 10


Dietary restriction (DR) increases life span and delays the onset of age-related diseases across species. However, the molecular mechanisms have remained relatively unexplored in terms of gene regulation. InC. elegans, a popular model for aging studies, the FOXA transcription factor PHA-4 is a robust genetic regulator of DR, although little is known about how it regulates gene expression. We profiled the transcriptome and miRNAome of an eat-2 mutant, a genetic surrogate of DR, by Next Generation  ...[more]

Similar Datasets

| S-EPMC5864837 | biostudies-literature
| S-EPMC2034646 | biostudies-literature
2008-12-15 | GSE9682 | GEO
| S-EPMC4208828 | biostudies-other
| S-EPMC1855296 | biostudies-other
| S-EPMC2824807 | biostudies-literature
2009-01-13 | E-GEOD-9682 | biostudies-arrayexpress
| S-EPMC7768346 | biostudies-literature
| S-EPMC4326946 | biostudies-literature
| S-EPMC6121374 | biostudies-literature