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Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.

ABSTRACT: BACKGROUND: In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N?=?2297) and Danish (N?=?134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR). FINDINGS: In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. CONCLUSIONS: Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.

SUBMITTER: Fischer M

PROVIDER: S-EPMC4247638 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.

Fischer Melina M Freuling Conrad M CM Müller Thomas T Schatz Juliane J Rasmussen Thomas Bruun TB Chriel Mariann M Balkema-Buschmann Anne A Beer Martin M Hoffmann Bernd B

Virology journal 20141125

<h4>Background</h4>In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).<h4>Findings</h4>In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequenc ...[more]

PMID: 25420461

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