Ontology highlight
ABSTRACT:
SUBMITTER: Villa-Diaz LG
PROVIDER: S-EPMC4250210 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Villa-Diaz Luis Gerardo LG Kim Jin Koo JK Lahann Joerg J Krebsbach Paul H PH
Stem cells translational medicine 20141013 12
We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of cont ...[more]