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Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.

ABSTRACT: We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.

SUBMITTER: Villa-Diaz LG

PROVIDER: S-EPMC4250210 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.

Villa-Diaz Luis Gerardo LG Kim Jin Koo JK Lahann Joerg J Krebsbach Paul H PH

Stem cells translational medicine 20141013 12

We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of cont ...[more]

PMID: 25313201

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