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Kocuria marina BS-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India.


ABSTRACT: Biosurfactant screening was made among the eight halophilic bacterial genera isolated from Kovalam solar salt works in Kanyakumari of India. After initial screening, Kocuria sp. (Km), Kurthia sp. (Ku) and Halococcus sp. (Hc) were found to have positive biosurfactant activity. Biosurfactant derived from Kocuria sp. emulsified more than 50% of the crude oil, coconut oil, sunflower oil, olive oil and kerosene when compared to the other strains. Further, Kocuria marina BS-15 derived biosurfactant was purified and characterized by TLC, FTIR and GC-MS analysis. The TLC analysis revealed that, the purified biosurfactants belong to the lipopeptide group. The IR spectrum results revealed that functional groups are R2C 000000000000 000000000000 000000000000 111111111111 000000000000 111111111111 000000000000 000000000000 000000000000 NN, alkenes and N-H. The GC-MS analysis confirmed the compound as Nonanoic acid and Cyclopropane with the retention time of 12.78 and 24.65, respectively.

SUBMITTER: Sarafin Y 

PROVIDER: S-EPMC4250494 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Kocuria marina BS-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India.

Sarafin Yesurethinam Y   Donio Mariathasan Birdilla Selva MB   Velmurugan Subramanian S   Michaelbabu Mariavincent M   Citarasu Thavasimuthu T  

Saudi journal of biological sciences 20140121 6


Biosurfactant screening was made among the eight halophilic bacterial genera isolated from Kovalam solar salt works in Kanyakumari of India. After initial screening, Kocuria sp. (Km), Kurthia sp. (Ku) and Halococcus sp. (Hc) were found to have positive biosurfactant activity. Biosurfactant derived from Kocuria sp. emulsified more than 50% of the crude oil, coconut oil, sunflower oil, olive oil and kerosene when compared to the other strains. Further, Kocuria marina BS-15 derived biosurfactant wa  ...[more]

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