Ontology highlight
ABSTRACT:
SUBMITTER: Bultema JB
PROVIDER: S-EPMC4250540 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
FEBS open bio 20141112
The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycoside hydrolase family 2 (GH2). Its commercial enzyme preparation, Biolacta N5, is used for commercial-scale production of galacto-oligosaccharides (GOS). The BgaD active site and catalytic amino acid residues have not been studied. Using bioinformatic routines we identified two putative catalytic glutamates and two highly conserved active site histidines. The site-directed mutants E447N, E532Q, and H ...[more]