Prognostic value of bone marrow involvement by clonal immunoglobulin gene rearrangements in follicular lymphoma.

ABSTRACT:

Aims

We aimed to evaluate the prognostic value of routine use of PCR amplification of immunoglobulin gene rearrangements in bone marrow (BM) staging in patients with follicular lymphoma (FL).

Methods

Clonal rearrangements were assessed by immunoglobulin heavy and light-chain gene rearrangement analysis in BM aspirates from 96 patients diagnosed with FL and related to morphological detection of BM involvement in biopsies. In 71 patients, results were also compared with concurrent flow cytometry analysis.

Results

BM involvement was detected by PCR in 34.4% (33/96) of patients. The presence of clonal rearrangements by PCR was associated with advanced clinical stage (I-III vs IV; p<0.001), high FL International Prognostic Index (FLIPI) score (0-1, 2 vs ≥3; p=0.003), and d

SUBMITTER: Berget E 

PROVIDER: S-EPMC4251203 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature