Unknown

Dataset Information

0

Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica.


ABSTRACT: To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. The successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism.

SUBMITTER: Wang W 

PROVIDER: S-EPMC4251831 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica.

Wang Wei W   Wei Hui H   Alahuhta Markus M   Chen Xiaowen X   Hyman Deborah D   Johnson David K DK   Zhang Min M   Himmel Michael E ME  

PloS one 20141202 12


To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain abl  ...[more]

Similar Datasets

| S-EPMC9252082 | biostudies-literature
| S-EPMC6346196 | biostudies-literature
| S-EPMC6282006 | biostudies-literature
| S-EPMC7761004 | biostudies-literature
2018-12-06 | GSE90993 | GEO
| S-EPMC8455373 | biostudies-literature
| S-EPMC5744042 | biostudies-literature
| S-EPMC7672257 | biostudies-literature
| S-EPMC95210 | biostudies-literature
| S-EPMC3957620 | biostudies-literature