Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line.
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ABSTRACT:
SUBMITTER: Rodrigues ES
PROVIDER: S-EPMC4255388 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
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Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line.
Rodrigues Evandra Strazza ES Picanço-Castro Virgínia V Espanhol Marta Regina MR de Andrade Luiz Alberto Martins LA Palma Patricia Vianna Bonini PV Kashima Simone S Fontes Aparecida Maria AM Covas Dimas Tadeu DT
SpringerPlus 20130718
Hek-293 cell line presents good production platform for recombinant therapeutic proteins, however little is known about the components that contribute to the cellular control of recombinant protein production. In this study, we generated a Hek-293 producing recombinant factor VIII (FVIII) and we evaluated the immunoglobulin-binding protein (BiP) and phytanoil-CoA α-hydroxylase (PAHX) expression levels which are known for diminishing FVIII production. Our analyses showed that the recombinant cell ...[more]
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