Unknown

Dataset Information

0

Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics.


ABSTRACT: One of the limiting factors in determining the sensitivity of tandem mass spectrometry using hybrid quadrupole orthogonal acceleration time-of-flight instruments is the duty cycle of the orthogonal ion injection system. As a consequence, only a fraction of the generated fragment ion beam is collected by the time-of-flight analyzer. Here we describe a method utilizing postfragmentation ion mobility spectrometry of peptide fragment ions in conjunction with mobility time synchronized orthogonal ion injection leading to a substantially improved duty cycle and a concomitant improvement in sensitivity of up to 10-fold for bottom-up proteomic experiments. This enabled the identification of 7500 human proteins within 1 day and 8600 phosphorylation sites within 5 h of LC-MS/MS time. The method also proved powerful for multiplexed quantification experiments using tandem mass tags exemplified by the chemoproteomic interaction analysis of histone deacetylases with Trichostatin A.

SUBMITTER: Helm D 

PROVIDER: S-EPMC4256517 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications


One of the limiting factors in determining the sensitivity of tandem mass spectrometry using hybrid quadrupole orthogonal acceleration time-of-flight instruments is the duty cycle of the orthogonal ion injection system. As a consequence, only a fraction of the generated fragment ion beam is collected by the time-of-flight analyzer. Here we describe a method utilizing postfragmentation ion mobility spectrometry of peptide fragment ions in conjunction with mobility time synchronized orthogonal ion  ...[more]

Similar Datasets

2015-08-18 | PXD000863 | Pride
| S-EPMC5461537 | biostudies-literature
| S-EPMC10284583 | biostudies-literature
| S-EPMC4000571 | biostudies-literature
| S-EPMC8176450 | biostudies-literature
| S-EPMC6812596 | biostudies-literature
| S-EPMC10126951 | biostudies-literature
| S-EPMC3633474 | biostudies-literature
| S-EPMC4082393 | biostudies-literature
| S-EPMC2830836 | biostudies-other