Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Z
PROVIDER: S-EPMC4256643 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Zhang Zhonghui Z Wu Elise E Qian Zhijian Z Wu Wen-Shu WS
Scientific reports 20141205
Stable and efficient knockdown of multiple gene targets is highly desirable for dissection of molecular pathways. Because it allows sequence-specific DNA binding, transcription activator-like effector (TALE) offers a new genetic perturbation technique that allows for gene-specific repression. Here, we constructed a multicolor lentiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of multiple gene targets. This platform is fully compatible with the Golden ...[more]