Browse
Submit Data
Databases
API
Help

Dataset Information

6 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium species related to M. leprae and M. lepromatosis from cows with bovine nodular thelitis.

ABSTRACT: Bovine nodular thelitis is a granulomatous dermatitis associated with infection with acid-fast bacteria. To identify the mycobacterium responsible for this infection, we conducted phylogenetic investigations based on partial sequencing of 6 genes. These bacteria were identified as an undescribed Mycobacterium species that was phylogenetically related to M. leprae and M. lepromatosis.

SUBMITTER: Pin D

PROVIDER: S-EPMC4257800 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Comparative sequence analysis of Mycobacterium leprae and the new leprosy-causing Mycobacterium lepromatosis.

Project description:Mycobacterium lepromatosis is a newly discovered leprosy-causing organism. Preliminary phylogenetic analysis of its 16S rRNA gene and a few other gene segments revealed significant divergence from Mycobacterium leprae, a well-known cause of leprosy, that justifies the status of M. lepromatosis as a new species. In this study we analyzed the sequences of 20 genes and pseudogenes (22,814 nucleotides). Overall, the level of matching of these sequences with M. leprae sequences was 90.9%, which substantiated the species-level difference; the levels of matching for the 16S rRNA genes and 14 protein-encoding genes were 98.0% and 93.1%, respectively, but the level of matching for five pseudogenes was only 79.1%. Five conserved protein-encoding genes were selected to construct phylogenetic trees and to calculate the numbers of synonymous substitutions (dS values) and nonsynonymous substitutions (dN values) in the two species. Robust phylogenetic trees constructed using concatenated alignment of these genes placed M. lepromatosis and M. leprae in a tight cluster with long terminal branches, implying that the divergence occurred long ago. The dS and dN values were also much higher than those for other closest pairs of mycobacteria. The dS values were 14 to 28% of the dS values for M. leprae and Mycobacterium tuberculosis, a more divergent pair of species. These results thus indicate that M. lepromatosis and M. leprae diverged approximately 10 million years ago. The M. lepromatosis pseudogenes analyzed that were also pseudogenes in M. leprae showed nearly neutral evolution, and their relative ages were similar to those of M. leprae pseudogenes, suggesting that they were pseudogenes before divergence. Taken together, the results described above indicate that M. lepromatosis and M. leprae diverged from a common ancestor after the massive gene inactivation event described previously for M. leprae.

| S-EPMC2747882 | biostudies-literature

Reverse vaccinology and subtractive genomics approaches for identifying common therapeutics against Mycobacterium leprae and Mycobacterium lepromatosis.

Project description:BackgroundMycobacterium leprae and Mycobacterium lepromatosis are gram-positive bacterial pathogens and the causative agents of leprosy in humans across the world. The elimination of leprosy cannot be achieved by multidrug therapy alone, and highlights the need for new tools and drugs to prevent the emergence of new resistant strains.MethodsIn this study, our contribution includes the prediction of vaccine targets and new putative drugs against leprosy, using reverse vaccinology and subtractive genomics. Six strains of Mycobacterium leprae and Mycobacterium lepromatosis (4 and 2 strains, respectively) were used for comparison taking Mycobacterium leprae strain TN as the reference genome. Briefly, we used a combined reverse vaccinology and subtractive genomics approach.ResultsAs a result, we identified 12 common putative antigenic proteins as vaccine targets and three common drug targets against Mycobacterium leprae and Mycobacterium lepromatosis. Furthermore, the docking analysis using 28 natural compounds with three drug targets was done.ConclusionsThe bis-naphthoquinone compound Diospyrin (CID 308140) obtained from indigenous plant Diospyros spp. showed the most favored binding affinity against predicted drug targets, which can be a candidate therapeutic target in the future against leprosy.

| S-EPMC8040911 | biostudies-literature

Early Human Migrations (ca. 13,000 Years Ago) or Postcontact Europeans for the Earliest Spread of Mycobacterium leprae and Mycobacterium lepromatosis to the Americas.

Project description:For over a century, it has been widely accepted that leprosy did not exist in the Americas before the arrival of Europeans. This proposition was based on a combination of historical, paleopathological, and representational studies. Further support came from molecular studies in 2005 and 2009 that four Mycobacterium leprae single-nucleotide polymorphisms (SNPs) and then 16 SNP subtypes correlated with general geographic regions, suggesting the M. leprae subtypes in the Americas were consistent with European strains. Shortly thereafter, a number of studies proposed that leprosy first came to the Americas with human migrations around 12,000 or 13,000 years ago. These studies are based primarily on subsequent molecular data, especially the discovery of a new leprosy species Mycobacterium lepromatosis and its close association with diffuse lepromatous leprosy, a severe, aggressive form of lepromatous leprosy, which is most common in Mexico and the Caribbean Islands. A review of these and subsequent molecular data finds no evidence for either leprosy species in the Americas before the arrival of Europeans, and strains of both species of leprosy found in eastern Mexico, Caribbean Islands, and Brazil came from Europe while strains found in western Mexico are consistent with their arrival via direct voyages from the Philippines.

| S-EPMC5700473 | biostudies-other

Mycobacterium lepromatosis

Project description:Mycobacterium lepromatosis overview

| PRJNA281018 | ENA

Diffuse Lepromatous Leprosy Due to Mycobacterium lepromatosis in Quintana Roo, Mexico.

Project description:A 43-year-old woman of Mayan origin from Quintana Roo, Mexico, was diagnosed with diffuse lepromatous leprosy. The etiologic bacillus was determined to be Mycobacterium lepromatosis instead of Mycobacterium leprae. This case likely represents the first report of this leprosy form and its agent in the southeastern tip of Mexico.

| S-EPMC4609715 | biostudies-literature

Detection of Mycobacterium lepromatosis in patients with leprosy in India [Retraction].

Project description:[This retracts the article on p. 1677 in vol. 11, PMID: 30349325.].

| S-EPMC6306048 | biostudies-literature

Detection of Mycobacterium lepromatosis in patients with leprosy in India.

Project description:IntroductionThe most commonly noted reactions in leprosy patients are type 1 reactions and erythema nodosum leprosum, with some rare phenomenon of host response known as Lucio phenomenon or leprosy of Lucio and Latapi which is caused by Mycobacterium lepromatosis. So far, no case of M. lepromatosis has been reported from India.Materials and methodsThe main objective of this study was to detect any positive cases of M. lepromatosis in India with such a complication. We screened slit skin smear/biopsy samples from lepromatous leprosy (LL) patients reporting to The Leprosy Mission Community Hospitals across the country. Eighty-eight slit skin smears were collected from leprosy patients in 70% ethanol. DNA was extracted from all these samples. Polymerase chain reaction (PCR) was done for 2 genes; one set was for 16S rRNA and the other set was for coproporphyrinogen III oxidase (hemN) gene. Then, sequencing was done for all positive amplicons. Homology of the sequences was analyzed using the Basic Local Alignment Search Tool at the National Center of Biotechnology Information database.ResultsAmong 88 isolates, we found 4 positive cases for M. lepromatosis. All 4 were LL cases with a bacteriological index ranging from 2+ to 4+. On the basis of the National Center of Biotechnology Information Basic Local Alignment Search Tool analysis, the sequenced amplicons of both genes matched with the M. lepromatosis 16S rRNA and phosphofructokinase genes but not with hemN gene of lepromatosis. This is the first report for the presence of M. lepromatosis in LL cases from India.ConclusionThis new species M. lepromatosis exists beyond Mexico, Singapore and it is the cause of DLL in India also. It may cause dual infections along with M. leprae in endemic areas like India.

| S-EPMC6188107 | biostudies-literature

Mycobacterium leprae

Project description:Transcriptome Profiling in Mycobacterium leprae. Implications for Type 2 Reactions (ENL) in Leprosy

| PRJNA339815 | ENA

Mycobacterium leprae

Project description:Mycobacterium leprae diversity and population dynamics in medieval Europe from novel ancient genomes

| PRJNA721828 | ENA

Mycobacterium leprae

Project description:Mycobacterium leprae sequencing from recurrent leprosy cases

| PRJNA328423 | ENA