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18F-FDG kinetics parameters depend on the mechanism of injury in early experimental acute respiratory distress syndrome.


ABSTRACT: PET with (18)F-FDG allows for noninvasive assessment of regional lung metabolism reflective of neutrophilic inflammation. This study aimed at determining during early acute lung injury whether local (18)F-FDG phosphorylation rate and volume of distribution were sensitive to the initial regional inflammatory response and whether they depended on the mechanism of injury: endotoxemia and surfactant depletion.Twelve sheep underwent homogeneous unilateral surfactant depletion (alveolar lavage) and were mechanically ventilated for 4 h (positive end-expiratory pressure, 10 cm H2O; plateau pressure, 30 cm H2O) while receiving intravenous endotoxin (lipopolysaccharide-positive [LPS+] group; n = 6) or not (lipopolysaccharide-negative group; n = 6). (18)F-FDG PET emission scans were then acquired. (18)F-FDG phosphorylation rate and distribution volume were calculated with a 4-compartment model. Lung tissue expression of inflammatory cytokines was measured using real-time quantitative reverse transcription polymerase chain reaction.(18)F-FDG uptake increased in LPS+ (P = 0.012) and in surfactant-depleted sheep (P < 0.001). These increases were topographically heterogeneous, predominantly in dependent lung regions, and without interaction between alveolar lavage and LPS. The increase of (18)F-FDG uptake in the LPS+ group was related both to increases in the (18)F-FDG phosphorylation rate (P < 0.05) and to distribution volume (P < 0.01). (18)F-FDG distribution volume increased with infiltrating neutrophils (P < 0.001) and phosphorylation rate with the regional expression of IL-1? (P = 0.026), IL-8 (P = 0.011), and IL-10 (P = 0.023).Noninvasive (18)F-FDG PET-derived parameters represent histologic and gene expression markers of early lung injury. Pulmonary metabolism assessed with (18)F-FDG PET depends on the mechanism of injury and appears to be additive for endotoxemia and surfactant depletion. (18)F-FDG PET may be a valuable imaging biomarker of early lung injury.

SUBMITTER: de Prost N 

PROVIDER: S-EPMC4258066 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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18F-FDG kinetics parameters depend on the mechanism of injury in early experimental acute respiratory distress syndrome.

de Prost Nicolas N   Feng Yan Y   Wellman Tyler T   Tucci Mauro R MR   Costa Eduardo L EL   Musch Guido G   Winkler Tilo T   Harris R Scott RS   Venegas Jose G JG   Chao Wei W   Vidal Melo Marcos F MF  

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 20141006 11


<h4>Unlabelled</h4>PET with (18)F-FDG allows for noninvasive assessment of regional lung metabolism reflective of neutrophilic inflammation. This study aimed at determining during early acute lung injury whether local (18)F-FDG phosphorylation rate and volume of distribution were sensitive to the initial regional inflammatory response and whether they depended on the mechanism of injury: endotoxemia and surfactant depletion.<h4>Methods</h4>Twelve sheep underwent homogeneous unilateral surfactant  ...[more]

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