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Transcriptional analysis of porcine circovirus-like virus P1.

ABSTRACT: Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts.The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids).We show a very complex viral transcription pattern in P1-infected cells.

SUBMITTER: Wen LB

PROVIDER: S-EPMC4258304 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Transcriptional analysis of porcine circovirus-like virus P1.

Wen Li-bin LB Wang Feng-zhi FZ He Kong-wang KW Li Bin B Wang Xiao-min XM Guo Rong-li RL Xie Jian-ping JP

BMC veterinary research 20141202

<h4>Background</h4>Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts.<h4>Results</h4>The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the v ...[more]

PMID: 25440084

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