Protein kinase D1 deficiency promotes differentiation in epidermal keratinocytes.

ABSTRACT:

Background

Protein kinase D (PKD or PKD1) is a serine/threonine protein kinase that has been shown to play a role in a variety of cellular processes; however, the function of PKD1 in the skin has not been fully investigated. The balance between proliferation and differentiation processes in the predominant cells of the epidermis, the keratinocytes, is essential for normal skin function.

Objective

To investigate the effect of PKD1 deficiency on proliferation and differentiation of epidermal keratinocytes.

Methods

We utilized a floxed PKD1 mouse model such that infecting epidermal keratinocytes derived from these mice with an adenovirus expressing Cre-recombinase allowed us to determine the effect of PKD1 gene loss in vitro. Proliferation and differentiation were monito

SUBMITTER: Choudhary V 

PROVIDER: S-EPMC4259831 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature