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Nucleotide sequence of conjugative prophage ?1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for e?ux resistance to macrolides in Streptococcus pyogenes.

ABSTRACT: Genetic element ?1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide e?ux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that ?1207.3 is 52,491 bp in length and contained 58 open reading frames (ORFs). A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. ?1207.3 codes for two different C-methylation systems, several phage structural genes, a lysis cassette (composed by a holin and a peptidoglycan hydrolase), and three site-specific resolvases of the serine recombinase family.

SUBMITTER: Iannelli F

PROVIDER: S-EPMC4260502 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Nucleotide sequence of conjugative prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for eﬄux resistance to macrolides in Streptococcus pyogenes.

Iannelli Francesco F Santagati Maria M Santoro Francesco F Oggioni Marco R MR Stefani Stefania S Pozzi Gianni G

Frontiers in microbiology 20141209

Genetic element Φ1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide eﬄux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that Φ1207.3 is 52,491 bp in length and contained 58 open reading frames (ORFs). A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. Φ1207.3 codes for two different C-methylati ...[more]

PMID: 25538698

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