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Evidence of feline panleukopenia infection in cats in India.

ABSTRACT: The samples collected from cats showing clinical signs suspected for feline panleukopenia infection were confirmed using various molecular techniques and virus isolation. The suspected samples were confirmed using feline parvovirus specific primers. The partial VP2 gene was submitted to GenBank for the first time in India (Accession number JQ684660.1). The PCR positive samples were further amplified using full length FPV VP2 gene specific primers and sequenced. The blast analysis revealed that the local field isolates of FPV showed 99 % homology with other FPV sequences available in the GenBank. The evidence for occurrence of feline panleukopenia infection in cats in Tamil Nadu, India was further confirmed by host specific nucleotides present in the VP2 gene region as well as virus isolation in A72 cell line.

SUBMITTER: Parthiban M

PROVIDER: S-EPMC4262318 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Evidence of feline panleukopenia infection in cats in India.

Parthiban Manoharan M Aarthi Komandur Seshadri KS Balagangatharathilagar Mani M Kumanan Kathaperumal K

Virusdisease 20141101 4

The samples collected from cats showing clinical signs suspected for feline panleukopenia infection were confirmed using various molecular techniques and virus isolation. The suspected samples were confirmed using feline parvovirus specific primers. The partial VP2 gene was submitted to GenBank for the first time in India (Accession number JQ684660.1). The PCR positive samples were further amplified using full length FPV VP2 gene specific primers and sequenced. The blast analysis revealed that t ...[more]

PMID: 25674629

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Project description:Forty diseased cats and seven healthy control cats from different sex, ages and breeds had examined clinically to confirm presence or absence of clinical symptoms of Feline panleukopenia disease (FP). Several tools including ELISA, gene expression analysis (qRT-PCR), DNA fragmentation test and apoptosis assay were conducted to determine the Feline panleukopenia disease in cat tissues. Clinical symptoms in the form of depression, fever, anorexia, vomiting, diarrhea, dehydration, anaemia and leucopenia were recorded in the diseased cats while no clinical sings were observed in control healthy cats. ELISA results showed that all of diseased (n?=?40) cats were positive while control cats (n?=?7) were negative for FP viral antigen. After carrying out of ELISA assay, supportive treatment trials including fluid therapy, immunostimulant, antibiotics to overcome dehydration, restoring electrolytes imbalances, combating secondary bacterial infection were conducted but all diseased cats were died and control cats exposed to soft death. Gene expression analysis detected high levels of FP viral gene in several cat tissues in which ilium exhibited high viral expression levels compared with jejunum. Also, viral expression levels in jejunum were higher than in mesenteric lymph nodes. In addition, viral expression levels were not detected in tissues of control cats. The results of the DNA fragmentation assay observed that DNA extracted from different tissues of infected cats exhibited damaged DNA bands as compared with DNA of control cats. DNA fragmentation rates in infected tissues increased significantly (P?<?0.01), the highest rates were showed in ilium and jejunum tissue than in mesenteric lymph nodes. Determination of apoptosis in cat tissues showed that rate of apoptosis/necrosis increased significantly (P?<?0.05) in infected cats tissues in comparison to control cats. Moreover the highest apoptotic ratios of infected cats were observed in ilium and jejunum tissues compared with mesenteric lymph nodes.

Dataset Information

Evidence of feline panleukopenia infection in cats in India.

Publications

Evidence of feline panleukopenia infection in cats in India.

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