A fine-scale phylogenetic analysis of free-living Burkholderia species in sugarcane field soil.
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ABSTRACT:
SUBMITTER: Tago K
PROVIDER: S-EPMC4262370 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
A fine-scale phylogenetic analysis of free-living Burkholderia species in sugarcane field soil.
Tago Kanako K Itoh Hideomi H Kikuchi Yoshitomo Y Hori Tomoyuki T Sato Yuya Y Nagayama Atsushi A Okubo Takashi T Navarro Ronald R Aoyagi Tomo T Hayashi Kentaro K Hayatsu Masahito M
Microbes and environments 20141120 4
The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10(7) g(-1) soil) ac ...[more]
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