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Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner.

ABSTRACT: The kinesin KIF1C is known to regulate podosomes, actin-rich adhesion structures that remodel the extracellular matrix during physiological processes. Here, we show that KIF1C is a player in the podosome-inducing signaling cascade. Upon induction of podosome formation by protein kinase C (PKC), KIF1C translocation to the cell periphery intensifies and KIF1C accumulates both in the proximity of peripheral microtubules that show enrichment for the plus-tip-associated proteins CLASPs and around podosomes. Importantly, without CLASPs, both KIF1C trafficking and podosome formation are suppressed. Moreover, chimeric mitochondrially targeted CLASP2 recruits KIF1C, suggesting a transient CLASP-KIF1C association. We propose that CLASPs create preferred microtubule tracks for KIF1C to promote podosome induction downstream of PKC.

SUBMITTER: Efimova N

PROVIDER: S-EPMC4265736 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner.

Efimova Nadia N Grimaldi Ashley A Bachmann Alice A Frye Keyada K Zhu Xiaodong X Feoktistov Alexander A Straube Anne A Kaverina Irina I

Journal of cell science 20141024 Pt 24

The kinesin KIF1C is known to regulate podosomes, actin-rich adhesion structures that remodel the extracellular matrix during physiological processes. Here, we show that KIF1C is a player in the podosome-inducing signaling cascade. Upon induction of podosome formation by protein kinase C (PKC), KIF1C translocation to the cell periphery intensifies and KIF1C accumulates both in the proximity of peripheral microtubules that show enrichment for the plus-tip-associated proteins CLASPs and around pod ...[more]

PMID: 25344256

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