Dataset Information

Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

ABSTRACT:

Objective

Pancreas-related complications are some of the most serious ones in abdominal surgery. The goal of this study was to develop and validate novel near-infrared (NIR) fluorophores that would enable real-time pancreas imaging to avoid the intraoperative pancreatic injury.

Design

After initial screening of a large NIR fluorophore library, the performance of 3 selected pancreas-targeted 700 nm NIR fluorophores, T700-H, T700-F, and MB, were quantified in mice, rats, and pigs. Dose ranging using 25 and 100 nmol, and 2.5 µmol of T700-F, and its imaging kinetics over a 4 h period were tested in each species. Three different 800 nm NIR fluorophores were employed for dual-channel FLARE™ imaging in pigs: 2 μmol of ZW800-1 for vessels and kidney, 1 μmol of ZW800-3C for lymph nodes, and 2 μmol of ESNF31 for adrenal glands.

Results

T700-F demonstrated the highest signal to background ratio (SBR), with peak SBR at 4 h postinjection in mice. In pigs, T700-F produced an SBR≥2 against muscle, spleen, and lymph nodes for up to 8 h after a single intravenous injection. The combination of T700-F with each 800 nm NIR fluorophore provided simultaneous dual-channel intraoperative imaging of pancreas with surrounding organs in real time.

Conclusion

Pancreas-targeted NIR fluorophores combined with the FLARE dual-channel imaging system enable the real-time intraoperative pancreas imaging which helps surgeons perform safer and more curative abdominal surgeries.

SUBMITTER: Wada H

PROVIDER: S-EPMC4265744 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

Wada Hideyuki H Hyun Hoon H Vargas Christina C Gravier Julien J Park GwangLi G Gioux Sylvain S Frangioni John V JV Henary Maged M Choi Hak Soo HS

Theranostics 20150101 1

<h4>Objective</h4>Pancreas-related complications are some of the most serious ones in abdominal surgery. The goal of this study was to develop and validate novel near-infrared (NIR) fluorophores that would enable real-time pancreas imaging to avoid the intraoperative pancreatic injury.<h4>Design</h4>After initial screening of a large NIR fluorophore library, the performance of 3 selected pancreas-targeted 700 nm NIR fluorophores, T700-H, T700-F, and MB, were quantified in mice, rats, and pigs. D ...[more]

PMID: 25553094

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Dataset Information

Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

Objective

Design

Results

Conclusion

Publications

Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

Similar Datasets

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