Dataset Information

Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011.

ABSTRACT:

Background

PorA, fetA and fHbp are three antigen encoding genes useful for meningococcal typing and FHbp is an important component of meningococcal B vaccines.

Methods

We performed sequence analysis of meningococcal porA, fetA and fHbp genes on 128 isolates from Western Australia, relating results to age, gender, race and geographic region.

Results

We found predominantly PorA subtypes P1.22,14-16 (n?=?23) and P1.7-2,4 (n?=?19); FetA subtypes F1-5 (n?=?41), F3-6 (n?=?11), F5-1 (n?=?10), F5-2 (n?=?9), F5-5 (n?=?8), F3-3 (n?=?8); and FHbp variant groups 1 (n?=?65) and 2 (n?=?44). PorA P1.22,14-16 and FHbp variant group 2 were associated with younger age and aboriginality.

Conclusions

FHbp modular groups of the bivalent recombinant FHbp vaccine and the multicomponent 4CMenB vaccine make up 8.3% and 47.7% respectively of the examined serogroup B isolates from 2000-2011, however to estimate vaccine efficacy requires an account of all vaccine antigens and their levels of expression.

SUBMITTER: Boan P

PROVIDER: S-EPMC4266217 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011.

Boan Peter P Metasan Norhaliza N Tempone Simone S Harnett Gerry G Speers David J DJ Keil Anthony D AD

BMC infectious diseases 20141212

<h4>Background</h4>PorA, fetA and fHbp are three antigen encoding genes useful for meningococcal typing and FHbp is an important component of meningococcal B vaccines.<h4>Methods</h4>We performed sequence analysis of meningococcal porA, fetA and fHbp genes on 128 isolates from Western Australia, relating results to age, gender, race and geographic region.<h4>Results</h4>We found predominantly PorA subtypes P1.22,14-16 (n = 23) and P1.7-2,4 (n = 19); FetA subtypes F1-5 (n = 41), F3-6 (n = 11), F5 ...[more]

PMID: 25495685

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Project description:Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). A recombinant vaccine called Bexsero® incorporates four subcapsular antigens (fHbp, NHBA, NadA and PorA) which are used to assign a Bexsero® antigen sequence type (BAST) to each meningococcal strain. The vaccine elicits an immune response against combinations of variants of these antigens which have been grouped into specific BAST profiles that have been shown to have different distributions within geographical locations thus potentially affecting the efficacy of the vaccine. In this study, invasive meningococcal disease isolates from the western seaboard of Australia (Western Australia; WA) were compared to those from the south-eastern seaboard (Victoria; VIC) from 2008 to 2012. Whole-genome sequencing (WGS) of 131 meningococci from VIC and 70 meningococci from WA were analysed for MLST, FetA and BAST profiling. Serogroup B predominated in both jurisdictions and a total of 10 MLST clonal complexes (cc) were shared by both states. Isolates belonging to cc22, cc103 and cc1157 were unique to VIC whilst isolates from cc60 and cc212 were unique to WA. Clonal complex 41/44 represented one-third of the meningococcal population in each state but the predominant ST was locally different: ST-6058 in VIC and ST-146 in WA. Of the 108 BAST profiles identified in this collection, only 9 BASTs were simultaneously observed in both states. A significantly larger proportion of isolates in VIC harboured alleles for the NHBA-2 peptide and fHbp-1, antigenic variants predicted to be covered by the Bexsero® vaccine. The estimate for vaccine coverage in WA (47.1% [95% CI: 41.1-53.1%]) was significantly lower than that in VIC (66.4% [95% CI: 62.3-70.5%]). In conclusion, the antigenic structure of meningococci causing invasive disease in two geographically distinct states of Australia differed significantly during the study period which may affect vaccine effectiveness and highlights the need for representative surveillance when predicting potential impact of meningococcal B vaccines.

Dataset Information

Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011.

Background

Methods

Results

Conclusions

Publications

Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011.

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