Modulation of DNA loop lifetimes by the free energy of loop formation.
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ABSTRACT:
SUBMITTER: Chen YJ
PROVIDER: S-EPMC4267329 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Modulation of DNA loop lifetimes by the free energy of loop formation.
Chen Yi-Ju YJ Johnson Stephanie S Mulligan Peter P Spakowitz Andrew J AJ Phillips Rob R
Proceedings of the National Academy of Sciences of the United States of America 20141119 49
Storage and retrieval of the genetic information in cells is a dynamic process that requires the DNA to undergo dramatic structural rearrangements. DNA looping is a prominent example of such a structural rearrangement that is essential for transcriptional regulation in both prokaryotes and eukaryotes, and the speed of such regulations affects the fitness of individuals. Here, we examine the in vitro looping dynamics of the classic Lac repressor gene-regulatory motif. We show that both loop assoc ...[more]
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