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Imaging mouse lung allograft rejection with (1)H MRI.

ABSTRACT: To demonstrate that longitudinal, noninvasive monitoring via MRI can characterize acute cellular rejection in mouse orthotopic lung allografts.Nineteen Balb/c donor to C57BL/6 recipient orthotopic left lung transplants were performed, further divided into control-Ig versus anti-CD4/anti-CD8 treated groups. A two-dimensional multislice gradient-echo pulse sequence synchronized with ventilation was used on a small-animal MR scanner to acquire proton images of lung at postoperative days 3, 7, and 14, just before sacrifice. Lung volume and parenchymal signal were measured, and lung compliance was calculated as volume change per pressure difference between high and low pressures.Normalized parenchymal signal in the control-Ig allograft increased over time, with statistical significance between day 14 and day 3 posttransplantation (0.046?0.789; P?

SUBMITTER: Guo J

PROVIDER: S-EPMC4272671 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Imaging mouse lung allograft rejection with (1)H MRI.

Guo Jinbang J Huang Howard J HJ Wang Xingan X Wang Wei W Ellison Henry H Thomen Robert P RP Gelman Andrew E AE Woods Jason C JC

Magnetic resonance in medicine 20140620 5

<h4>Purpose</h4>To demonstrate that longitudinal, noninvasive monitoring via MRI can characterize acute cellular rejection in mouse orthotopic lung allografts.<h4>Methods</h4>Nineteen Balb/c donor to C57BL/6 recipient orthotopic left lung transplants were performed, further divided into control-Ig versus anti-CD4/anti-CD8 treated groups. A two-dimensional multislice gradient-echo pulse sequence synchronized with ventilation was used on a small-animal MR scanner to acquire proton images of lung a ...[more]

PMID: 24954886

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