Ontology highlight
ABSTRACT:
SUBMITTER: Jensik PJ
PROVIDER: S-EPMC4274154 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Jensik Philip J PJ Vargas Jesse D JD Reardon Sara N SN Rajamanickam Shivakumar S Huggenvik Jodi I JI Collard Michael W MW
PloS one 20141222 12
DEAF1 is a transcriptional regulator associated with autoimmune and neurological disorders and is known to bind TTCG motifs. To further ascertain preferred DEAF1 DNA ligands, we screened a random oligonucleotide library containing an "anchored" CpG motif. We identified a binding consensus that generally conformed to a repeated TTCGGG motif, with the two invariant CpG dinucleotides separated by 6-11 nucleotides. Alteration of the consensus surrounding the dual CpG dinucleotides, or cytosine methy ...[more]