Ontology highlight
ABSTRACT:
SUBMITTER: Doamekpor SK
PROVIDER: S-EPMC4274631 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Doamekpor Selom K SK Schwer Beate B Sanchez Ana M AM Shuman Stewart S Lima Christopher D CD
RNA (New York, N.Y.) 20141120 1
mRNA capping enzymes are directed to nascent RNA polymerase II (Pol2) transcripts via interactions with the carboxy-terminal domains (CTDs) of Pol2 and transcription elongation factor Spt5. Fission yeast RNA triphosphatase binds to the Spt5 CTD, comprising a tandem repeat of nonapeptide motif TPAWNSGSK. Here we report the crystal structure of a Pct1·Spt5-CTD complex, which revealed two CTD docking sites on the Pct1 homodimer that engage TPAWN segments of the motif. Each Spt5 CTD interface, compo ...[more]